Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, pH 7.6) - 4L
Phosphate Buffered Saline PBS (10X pH 7 6) - 4L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffer (1 M, pH 8.5) - 500ml
HEPES Buffer (1 M pH 8 5) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc PIPES Buffer (1 M, pH 7.0) - 500ml
PIPES Buffer (1 M pH 7 0) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Sodium Phosphate Buffer (0.2 M, pH 7.4) - 500ml
Sodium Phosphate Buffer (0 2 M pH 7 4) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, with Triton X-100) - 1L
Phosphate Buffered Saline PBS (10X with Triton X-100) - 1L (For Research Use and Further Manufacturing Only)
IBA LifeSciences Buffer XT-R - Strep-TactinXT regeneration buffer; 250 ml
Buffer XT-R is a ready-to-use Strep-TactinXT regeneration buffer containing magnesium chloride for competitive displacement of biotin from the modified biotin-binding pocket of Strep-TactinXT after the elution of Strep-tagII or Twin-Strep-tag fusion proteins. Please note that Buffer XT-R is not applicable for Strep-Tactin.
LIFE TECH (PPA ECHO ONLY) TRIS HYDRMETH AMINOMETHANE HCL
NC3798150 TRIS HYDRMETH AMINOMETHANE HCL
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4040864 BUFFERED AQUEOUS GLYCEROL SOLU
VWR INTERNATIONAL LLC PHOSPHATE BUFFERD SALINE 500ML
PHOSHPHATE BUFFERED SALINE (PBS), STERILE LIQUID. 500ml. 1X Sterile PBS, pH 7.4. 137 mM sodium chloride, 2.7 mM potassium chloride, 12 mM phosphate buffer.
Apexbio Technology LLC EdU Flow Cytometry Assay Kits (HF488) 100tests
EdU Flow Cytometry Assay Kits (HF488) provides a novel method based on click chemistry specifically CuAAC (copper-catalyzed azide-alkyne cycloaddition) enabling direct measurement of DNA synthesis during the S-phase of the cell cycle The thymidine analog EdU (5-ethynyl-2 -deoxyuridine) can be incorporated directly into DNA strands during DNA synthesis The alkyne group on EdU a biologically inert moiety undergoes highly selective reaction with azide-functionalized dyes via the CuAAC reaction forming a stable 1 2 3-triazole product The EdU Flow Cytometry Assay Kits (HF488) use HyperFluor 488 azide to label the DNA of proliferating cells following its reaction with EdU Samples labeled in this manner can be analyzed via flow cytometry (HyperFluor 594 azide excitation maximum at 496 nm emission maximum at 516 nm)